Fig. 1

Overview of the main concepts and messages of the paper. The chemiluminescence signal (C) is generated from the solution of BSA (A) when exposed to a sequence of intense electric pulses (B). No external chemiluminescent labels are required; the signal arises due to components endogenous to the sample. The chemiluminescence reports on oxidative processes in the BSA solution. We analyzed the behavior of chemiluminescence in the presence or absence of prooxidant as well as enzymatic antioxidants and respective combinations. Furthermore, we used various biochemical and biophysical techniques (D) to analyze the effects of the PEF treatment on BSA.