Fig. 2 | Scientific Reports

Fig. 2

From: Giant pyramidal neurons of the primary motor cortex express vasoactive intestinal polypeptide (VIP), a known marker of cortical interneurons

Fig. 2

Expression of vasoactive intestinal polypeptide (VIP) is limited to the largest layer 5 pyramidal cells. Representative images of Area 4c (a–e) and A6DC (caudal subdivisions of the dorsal premotor area, f–j) are shown using myelin (a,f), Nissl (b and g) and VIP staining (c and h) in CJ226. Arrowheads point to the areal boundaries5. Red lines in (a–c and f–h) identify layers 2–6, while layer 5 is indicated by small black lines. Dashed rectangles in (b,c,g,h) are shown at higher magnification in (d,e,i,j), respectively. Large pyramidal cells are visible within layer 5 (between the two dashed lines) only in Nissl staining (d,i). Scale 1 mm for (a–c) and (f–h), 200 µm for (d–e) and (i–j). VIP antibody used in production of images is from Abcam (Cat# ab30680).

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