Table 2 List of primary and secondary antibodies used for immunostaining.
Staining method | Primary antibody | Source and identifier | Secondary antibody | Source and identifier |
|---|---|---|---|---|
Parvalbumin (PV)-IHC | Mouse monoclonal (1:8000) | Swant Cat# 235; PRID: AB_10000343 | Secondary antibody (Mouse IgG, 1:200) and Vectastain elite ABC HRP kit; DAB substrate kit | Vector laboratories Cat# PK-6102; RRID: AB_2336821; vector laboratories Cat# SK-4100; RRID: AB_2336382 |
Calbindin-D28k (CB)-IHC | Mouse monoclonal (1:8000) | Swant Cat# 300; RRID: AB_10000347 | Same as above | Same as above |
Calretinin (CR)-IHC | Mouse monoclonal (1:8000) | Swant Cat# 6B3; RRID: AB_10000320 | Same as above | Same as above |
Neuronal marker (NeuN)-IHC | Mouse monoclonal (1:800) | Millipore Cat# MAB377; Clone A60; RRID: AB_2298772 | Same as above | Same as above |
Vasoactive intestinal polypeptide (VIP)-IHC | Mouse monoclonal (1:500) | Abcam Cat# ab30680; RRID: AB_778830 | Same as above | Same as above |
VIP-IF | Rabbit polyclonal (1:200) | Atlas antibodies Cat# HPA017324; RRID: AB_1858754 | Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (1:600) | Abcam Cat# ab150064; RRID: AB_2734146 |
VIP-IF | Mouse monoclonal (1:500) | NeuroMab Cat# L108/92; PRID: AB_2651163 | Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:600) | Abcam Cat# ab150109 RRID: AB_2571721 |
GABA-IF | Rabbit polyclonal (1:500) | Sigma-Aldrich Cat# A2052; RRID: AB_477652 | Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (1:600) | Abcam Cat# ab150064; RRID: AB_2734146 |
PV-IF | Mouse monoclonal (1:500) | Swant Cat# 235; PRID: AB_10000343 | Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:600) | Abcam Cat# ab150109 RRID: AB_2571721 |
NeuN-IF | Rabbit polyclonal (1:700) | Millipore Cat# ABN78; RRID: AB_10807945 | Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (1:600) | Abcam Cat# ab150064; RRID: AB_2734146 |
