Fig. 3
From: Variant load of mitochondrial DNA in single human mesenchymal stem cells
Somatic heteroplasmic count and heteroplasmic fractions stratified by locus. Strip charts showing count of heteroplasmies (HF > 0.02/< 0.98) per cell per sample after normalisation by locus length (total region bp lengths: D-loop = 1124, rRNA = 2511, tRNA = 1486 and Protein Coding = 11,382) (left) and the heteroplasmic fraction (HF > 0.02/< 0.98) of somatic heteroplasmies (right) grouped by locus type. As expected, there is a higher frequency of heteroplasmic variants in the D-Loop compared to rRNA, tRNA and protein-coding regions (ANOVA p = 8.2 × 10–5, Dunnett’s p, D-Loop v rRNA = 0.002, D-Loop v tRNA = 0.011, and D-Loop v protein coding = 0.001). There was no significant difference in overall HF between loci (ANOVA p = 0.115), However, rRNA HFs appear higher when compared to D-Loop as a reference (Dunnett’s p, D-Loop v rRNA = 0.038). tRNA and protein HFs distributions were not significantly different (Dunnett’s p, D-Loop v tRNA = 0.203 and D-Loop v protein coding = 0.082).
