Fig. 4
Generation of liver-specific CAR knockout mice and liver protein expression. (A) CAR deletion confirmed liver of 8-weeks-old WT and KO mice by immunofluorescence stain and western blot analysis. (B) Protein was extracted from the livers of WT and KO mice and then subjected to western blot analysis using anti-CAR, -APOBEC3C, and -LDLR antibodies (n = 4 for each group). CAR deletion altered lipid transport regulatory proteins and gene expression in the isolated hepatocytes. (C) The liver hepatocytes of WT and KO mice were isolated and then subjected to a lipid uptake assay. Oil Red O staining showed lipid uptake levels in the hepatocytes. (D) APOBEC3C, ApoB, and LDLR mRNA levels were measured in WT and KO hepatocytes by qPCR. Data are expressed as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001.
