Fig. 5

HFD-induced non-alcoholic fatty liver diseases in liver-specific CAR KO mice. (A) The basal phenotype of liver-specific CAR KO mice was confirmed. Body and liver weight were measured under different diet conditions for 16 weeks in pair-fed (■ WT and □ KO) or HFD (◆ WT and ◇ KO) (n = 4 each group) mice. (B) Concentrations of ALT, AST, total cholesterol, and triglyceride (TG) in serum were measured using ELISA (n = 4 in each group). (C,D) After HFD for 16 weeks, the liver was subjected to histological analysis by H&E and neutral lipid Lipo-Tox staining. Peritoneal fat was observed in the paraffin section. Lipid droplets increased in KO mice compared to WT fed an HFD. However, there was no difference between pair-fed mice. Data are expressed as mean ± SD, *P < 0.05 by a two-tailed Student’s t-test.