Fig. 6
HFDs induced inflammatory cytokine expression and cholesterol accumulation in the liver of KO mice. (A) Liver protein was extracted and then subjected to ELISA. The total cholesterol, triglyceride, and LDL cholesterol levels were measured. (B) Total RNA was extracted from mice liver after being pair-fed or fed an HFD and then subjected to qPCR analysis of ApoB and APOBEC3C. In addition, (C) inflammatory cytokines (IL-1β, IL-6, TNF-α, and MCP-1) and immunocytes (F4/80: macrophage marker; n = 4). (D) Liver subjected to immunofluorescent stain for macrophages. Liver samples were incubated with anti-F4/80 antibodies and Alexa488-labeled secondary antibodies. Infiltrated macrophages presented are by green stain percent area. Data are expressed as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001 by a two-tailed Student’s t-test. IL-1β interleukin-1 beta; IL-6, interleukin-6; TNF-α tumor necrosis factor alpha, MCP-1 monocyte chemoattractant protein-1.
