Fig. 1
From: Faster ciguatoxin extraction methods for toxicity screening
Flow diagram of the batch liquid‒liquid extraction and full clean-up methods. The acetone method was developed by Dickey27 and modified herein; the quick enzyme and full enzyme methods were developed by Spielmeyer et al.45. Fish tissue used was 1 g dry or 5 g wet tissue equivalent (eq.). Tissue to solvent volume ratios are described in the ‘Sample extraction’ section of the respective methods. Final residues were reconstituted in methanol for analysis.
