Fig. 2
From: Faster ciguatoxin extraction methods for toxicity screening
Combined concentration‒response curves from the cell-based assay derived without the addition of ouabain (O) or veratridine (V) (– OV, solid symbols) and with the addition of OV (+ OV, open symbols) when exposed to various concentrations of semipurified fish extracts. The cells were exposed to 0.22 and 0.022 mM O/V, respectively. Species are listed from top to bottom: E. canina (mg DTE), S. barracuda (mg DTE), and Lutjanus spp. (mg WTE). The extraction methods used were as follows (from left to right): acetone (ACT), methanol (MeOH), and quick-enzyme (Q-Enz). Each graph represents an average of n = 3 independent 96-well plates per extract (see Table 2 for individual results in the CTX-1B eq.). Error bars represent the standard deviation from all independent 96-well plate analyses performed for each sample (minimum of three independent 96-well plates; each assay included three replicate points).
