Table 1 Handling steps and average EC50 (mg tissue eq.) for each method.
From: Faster ciguatoxin extraction methods for toxicity screening
Steps | Method | |||
|---|---|---|---|---|
Acetone | Methanol | Quick-enzyme | Full-enzyme | |
Ultra Turrex | 1 | 1 | 0 | 0 |
Cooking | 0 | 0 | 1 | 1 |
EnzymATIC degradation | 0 | 0 | 1 | 1 |
Vortex | 5 | 5 | 6 | 8 |
Tube transfer | 4 | 4 | 3 | 3 |
Centrifugation | 7 | 7 | 3 | 5 |
Drying | 3 | 1 | 2 | 4 |
Solid phase extraction | – | – | – | 2 |
Total steps | 20 | 18 | 16 | 24 |
Average EC50* (mean tissue eq. ± standard error of the mean) | 0.101 ± 0.010 (n = 24) | 0.091 ± 0.009 (n = 27) | 0.077 ± 0.009 (n = 27) | 0.128 ± 0.023 (n = 27) |
