Table 1 Chronic unpredictable mild stress procedure.
No | Stressor | Definition |
|---|---|---|
① | Immobilization | The rat was placed in a rat restrainer and restrained alone for 2 h |
② | Empty cage | The rat was confined in a cage without any bedding for 24 h |
③ | Cold water swimming | The rat was placed in a cylindrical clear plastic container filled with 6 ℃ water for 5 min |
④ | Wet padding material | The rat was placed in a cage with 200 ml water in 100 g sawdust for 24 h |
⑤ | Clip the tail | The rat was pinched for one minute at a distance of 3 cm from the tail |
⑥ | Fast for 24 h | The rat was subjected to 24 h of food deprivation |
⑦ | Continuous light | The rat was exposed to continuous light for 24 h |
⑧ | Cage tilt | The rat cage was tilted 45 degrees for 24 h |
⑨ | Strong cage shaking | The rat rocked back and forth for 15 min |
⑩ | Thermal stimulus | The rat was placed in a drying oven at 45 °C for 15 min |
