Fig. 4 | Scientific Reports

Fig. 4

From: CD8 + T-cells restrict the development of peritoneal metastasis and support the efficacy of hyperthermic intraperitoneal chemotherapy (HIPEC)

Fig. 4

Induction of antigen-specific CD8 + T cells via heated chemotherapy. (a)Time-line of the experiment. (b) flow cytometry data on the maturation state of Mo-DC`s depending on the cancer-cell treatment. (c)Time-line of the experiment with additional co-culture of CD8 + T-cells. (d) Shows flow cytometry data of CD8 + T-cells and their IFN-γ production depending on the cancer-cell treatment. (e) Shows the time-line of a similar experiment using splenocytes from OT-I mice, which have a specific TCR for the ovalbumin. (f) Presents the ratio of IFN-γ positive CD8 + T-cells after co-culturing with PBS, or M/D or heated M/D treated MC-38-Ova cancer cells with splenocytes from a OT-I mouse. Cancer cell killing by specific CD8 + T-cells. (g) time-line of the co-culture experiment. The % of cancer cell killing by the treatment with or without specific CD8 + T-cells is shown in (h).The experiments were performed in triplicates. Error bars show the mean +/-SD. **** = p ≤ 0.0001, *** = p ≤ 0.001, ** == p ≤ 0.01, * = p ≤ 0.05, ns = p > 0.05.

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