Fig. 2
Transcriptomic analysis reveals JAK/STAT signaling pathway as a hub in MSC capillary-like structure formation. Human mesenchymal stromal/stem cells (MSC) were seeded as either cell monolayers or as capillary-like structures on top of Cultrex for 4 h. Total RNA was extracted from triplicate samples for each condition, and gene expression modulation was assessed through RNA-Seq analysis as described in the Methods section. (A) Network graph showing enriched pathways and their respective differentially expressed genes (DEGs) in cells cultured on Cultrex versus cells cultured as monolayers. Upregulated and downregulated genes are color-coded in green and red, respectively. (B) KEGG pathways enrichment analysis of DEGs with an absolute fold change (FC) > 2 and adjusted p-value < 0.001 in each experimental condition68,69,70. Data was analyzed on triplicate samples from a representative experiment.
