Fig. 4
Effects of QR-KLU on cell migration, single-cell proliferation, and the expression of HIF-1α and VEGF (n = 6). (A) Effects of KLU and QR-KLU on the migration ability of Hepa1–6 cells after 24 and 48 h of cultivation under normoxic conditions. Hypoxic condition was created by adding 100 µM cobalt dichloride. (B) Effects of KLU and QR-KLU on the migration ability of Hepa1–6 cells after 24 and 48 h of cultivation under hypoxic conditions. (C) Representative images of the plate clone of Hepa1–6 cells treated with KLU and QR-KLU under normoxic and hypoxic conditions. (D) Representative images of the expression of HIF-1α and VEGF in Hepa1–6 cells treated with PBS and QR-KLU under normoxic and hypoxic conditions. HIF-1α; PBS phosphate-buffered saline, QR-KLU peptide (QR)–drug (KLU) conjugate, VEGF vascular endothelial growth factor.
