Fig. 2
SnRNA-seq of 68,775 nuclei from female and male mouse kidneys at 1, 2 ,4 and 12 weeks of age. (A) Results of cell clustering and cell type identification are shown using uniform manifold approximation and projection (UMAP) plot. Expected major types of kidney cells and their respective proliferative cell clusters were identified. (B) Dotplot shows the expression levels and the percentage of gene expression of the canonical genes in each cluster. (C) Results of cell cycle analysis. (D) Feature plot shows cells that express the Xist gene, which is specific for female cells. Regional expression of Xist is noted in PTCs but not in other cell types on the UMAP. (E–G) Evolutional changes of the percentage of each cell type at different ages. The percentage of proliferative cells, EC, fib, JGC, MC, Pod decreased with age, whereas the percentage of tubular cells increased with age. (H) Percentage of proliferating cells relative to the corresponding cell type rather than total cells in the kidney. Pod, podocyte; EC, endothelial; Prolif_EC, proliferative endothelial cells; MC, mesangial cell; JGC, Juxtaglomerular cells; PT, proximal tubule; S1/S2/S3, segment 1/2/3 of proximal tubule; Prolif_PT, proliferative proximal tubule; PTdediff_PTC, dedifferentiated proximal tubule_parietal cell; DTL, descending thin limb; ATL, ascending thing limb; TAL, thick ascending limb; Prolif_TAL, proliferative thick ascending limb; TAL_DCT, thick ascending limb_ distal convoluted tubule; DCT1/DCT2, distal convoluted tubule1/2; CNT, connecting tubule; PC_OMCD, principal cell-outer medullary collecting duct; PC_IMCD, principal cell-inner medullary collecting duct; IC_A, intercalated cells, type A; IC_B, intercalated cells, type B; Fib, fibroblast; Prolif_Fib, proliferative fibroblast; Uro, urothelial cell.
