Fig. 3
Validation of the snRNA-seq result using immunofluorescence stain of the proliferative cells in S1, S2 and S3 segments of the proximal tubule. Immunofluorescence stain shows the colocalization of the proliferative marker Ki67 and the PTC markers for S1 segment, SLC5A2 (A); S2 segment, SLC13A3 (B) and S3 segment, SLC5A10 (C) (scale = 20 μm). Proliferating cells were identified in S1, S2 and S3 segments at 1, 2, 4, and 12 weeks-old male and female mice. The image is representative of 2 mice in each group. High proliferation was noted postnatally and reduced as the tissue matures in all three PT segments under microscope.
