Fig. 6

Correlations of TAMs with the plasma concentration of 25(OH)D₃ and the expression of CYP27B1 in tumor tissue. (A) Spearman r correlations of 25(OH)D₃ [ng/mL] with CD80; N = 16. Spearman r correlations of 25(OH)D₃ with (B) CD200R (all patients: N = 42, VD-deficient: N = 31, premenopausal: N = 15, nonmetastatic: N = 26), (C) CD80 (N = 16), (D) CD204 (postmenopausal: N = 27, nonmetastatic: N = 26), (E) CD44 (postmenopausal: N = 27, nonmetastatic: N = 26), and F HLA-DR (premenopausal: N = 15, nonmetastatic: N = 26). CYP27B1, 1α-hydroxylase; VD, vitamin D₃; The expression of TAMs markers were analyzed using the BD FACS Diva™ 6.2 program, and the fluorescence intensity was determined relative to that of the unstained control (MFI). The expression of CYP27B1 was analyzed by western blot and CYP27B1/β-actin ratio are determined. *P < 0.05, **P < 0.01, ***P < 0.001.