Table 4 Rabbit monoclonal and polyclonal antibodies used for protein immunodetection.
From: Vitamin D3 reduces the expression of M1 and M2 macrophage markers in breast cancer patients
Protein | Clone/catalog number | Dilution* |
|---|---|---|
EpCAM, epithelial cell adhesion molecule antigen | 21050-1-AP | 1:1000 |
OPN, osteopontin | 22952-1-AP | |
ZEB-1, proteins from the E-Box zinc finger family binding Homeobox 1 | 21544-1-AP | 1:1000 |
VDR, vitamin D receptor | D2K6W/#12,550 | |
CYP24A1, 24-hydroxylase | H-87/#sc-66,851 | |
CYP27B1, 1α-hydroxylase | EPR20271/ab206655 | |
MMP-3, metalloproteinase 3 | JM46-22/NBP2-75931 | |
IRF4, interferon regulatory factor 4 | 11247-2-AP | 1:500 |
TGFβ, transforming growth factor β | 21898-1-AP | |
S100A8, S100 calcium-binding protein A8 | 15792-1-AP | 1:1000 |
PTHLH, parathyroid hormone-like hormone | NBP3-03168 | |
Vimentin | NBP1-31327 | 1:5000 |