Table 4 Rabbit monoclonal and polyclonal antibodies used for protein immunodetection.

From: Vitamin D3 reduces the expression of M1 and M2 macrophage markers in breast cancer patients

Protein

Clone/catalog number

Dilution*

EpCAM, epithelial cell adhesion molecule antigen

21050-1-AP

1:1000

OPN, osteopontin

22952-1-AP

 

ZEB-1, proteins from the E-Box zinc finger family binding Homeobox 1

21544-1-AP

1:1000

VDR, vitamin D receptor

D2K6W/#12,550

 

CYP24A1, 24-hydroxylase

H-87/#sc-66,851

 

CYP27B1, 1α-hydroxylase

EPR20271/ab206655

 

MMP-3, metalloproteinase 3

JM46-22/NBP2-75931

 

IRF4, interferon regulatory factor 4

11247-2-AP

1:500

TGFβ, transforming growth factor β

21898-1-AP

 

S100A8, S100 calcium-binding protein A8

15792-1-AP

1:1000

PTHLH, parathyroid hormone-like hormone

NBP3-03168

 

Vimentin

NBP1-31327

1:5000

  1. *The antibodies were diluted in TBST solution supplemented with blocking solution (10:1).