Fig. 2 | Scientific Reports

Fig. 2

From: Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs

Fig. 2

VH, VLλ, and VLκ amplification via conventional PCR. (a) VLκ amplicons were amplified via sets of forward primers (CSCK1-F, 24-F, 34-F, 4-F, 5-F, and 6-F; numbers 1, 24, 34, 4, 5, and 6 in the figure). Four reverse primers were used: CSCJK1-B, CSCJK2-B, CSCJK3-B, and CSCJK4-B. (b) VLλ amplicons were amplified via sets of forward primers (CSCLam1a-F–CSCLam13-F; numbers 1a, 1b, 1c, 1d, and 2–13 in the figure). CSCJLam1-B and CSCJLam2-B served as reverse primers. The size of the VLκ and VLλ PCR products was 350 bp. (c) VH amplicons were amplified via the forward primer (CSCVH1-F–CSCVH9-F; numbers 1–9 in the figure) and the CSCG1234-B reverse primer. The size of the VH PCR products was 450 bp. (d) Purified variable chain fragments. After the antibody fragments were amplified via PCR, the corrected PCR products were purified via gel extraction (Lane 1, purified VH; Lane 2, purified VLλ; and Lane 3, purified VLκ). The PCR products were run on 1.2% agarose gels.

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