Fig. 3

Colony PCR of 16 randomly selected clones from the (a) VHVLκ scFv library and (b) VHVLλ scFv library. The inserted scFv antibodies were amplified via second-round PCR primers (RSC-F and RSC-B(AA) primers), and the size of the PCR product was 850–900 bp, as observed on a 1% agarose gel. M refers to the 100 bp DNA ladder.