Fig. 6 | Scientific Reports

Fig. 6

From: Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs

Fig. 6

ELISA absorbance signal at 450 nm from soluble fractions of all 37 clones. Each sample consisted of four wells: expression control, RABV, rG, and negative control (SM). (a) Clone numbers 1–19. (b) Clone numbers 20–37. (c) Clone numbers 38–58. Differences between the absorbance levels of the target antigens and SM binding were assessed via independent t tests. The asterisks indicate significant differences as follows: P value ≤ 0.05 (*), ≤ 0.01 (**), and ≤ 0.001 (***). The symbol “#” refers to an excluded sample whose signal from the negative control was greater than or equal to that of the scFv samples. A secondary anti-HA-conjugated HRP antibody bound to the scFv was detected using a TMB substrate. The error bars indicate the SDs of two replicates.

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