Table 1 Sequence diversity of the five selected K9RABVscFv fragments compared with the ig germline IMGT database.
K9RABV clone | Antibody type | CDR3 AA sequence | Germline | Family | V-gene | |
|---|---|---|---|---|---|---|
scFv1 | Full scFv (VHVLҡ) | VLҡ: | GQGTHFPWT | IGKV2-10*01 | IGKV2 | IGKV2-10 |
VH: | AREAYMGLYNFDY | IGHV3-5*01 | IGHV3 | IGHV3-5 | ||
scFv2 | Full scFv (VHVLҡ) | VLҡ: | GQGTHDPWT | IGKV2-5*02 | IGKV2 | IGKV2-5 |
VH: | AASIEY | IGHV3-5*01 | IGHV3 | IGHV3-5 | ||
scFv12 | Full scFv (VHVLҡ) | VLҡ: | GQGIQDPPGT | IGKV2-6*01 IGKV2-9*01 | IGKV2 | IGKV2-6 IGKV2-9 |
VH: | GDSWPLDS | IGHV3-5*01 | IGHV3 | IGHV3-5 | ||
scFv15 | Full scFv (VHVLλ) | VLλ: | GQNLQFPYT | IGLV2-5*02 | IGLV2 | IGLV2-5 |
VH: | ANHPYPVATDPFDH | IGHV3-6*01 | IGHV3 | IGHV3-6 | ||
scFv16 | Full scFv (VHVLҡ) | VLҡ: | GQGVQFPRT | IGKV2-5*02 | IGKV2 | IGKV2-5 |
VH: | ARDVNYYISDY | IGHV3-41*01 | IGHV3 | IGHV3-41 |
