Table 2 The RFFIT neutralizing activity of seven purified K9RABVscFv samples.

From: Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs

scFv

Concentration (mg/ml)

Neutralizing activity

RFFIT Titer (IU/ml)

> Cutoff value

at 0.5 IU/ml

K9RABVscFv1_1

K9RABVscFv1_2*

5.35

7.35

Positive

Positive

3.51

4.96

✓

✓

K9RABVscFv2

6.12

Negative

-

–

K9RABVscFv12

4.08

Negative

-

–

K9RABVscFv15

4.30

Negative

-

–

K9RABVscFv16_1

K9RABVscFv16_2*

5.88

7.11

Positive

Positive

2.37

5.65

✓

✓

Control

Concentration (mg/ml)

Neutralizing activity

RFFIT Titer (IU/ml)

> Cutoff value

at 0.5 IU/ml

1 IU RAI

6.7

Positive

1

✓

ERIG 1

ERIG 2

0.17

1.70

Positive

Positive

1

10

✓

✓

Negative control

–

Negative

0

–

  1. A total of 8 fields per well were observed. Only negative fields were counted.
  2. RAI = Human rabies immunoglobulins (reference standard).
  3. ERIG = TRCS® Equine Rabies Immunoglobulins (as an internal control).
  4. Negative control = serum from healthy, RABV-unvaccinated individuals.
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