Table 4 The set of primers based on the pComb3XSS phagemid for canine VH and VL chains and scFv amplification included sequencing primers according to Braganza et al.. (2011)36. Highlights refer to adjusted nucleotide bases in the primers.

From: Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs

Primer name

Sequence (5’–3’)

First round PCR primers

Canine VH forward primers

CSCVH1-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAG GTV CAR CTG GTG SAR TCT

CSCVH2-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAG GTR MVD YTG GTG GAR TCT

CSCVH3-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GRS GTG CAG CTG GTG GAG TCT

CSCVH4-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAG GTR CAG CTG STG GAG WMT

CSCVH5-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAR KWG CAR CTG GTG GAG YTT

CSCVH6-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAG GGG CAG CTG GCG GAG TCT

CSCVH7-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAR BTN MAR YTG GTN GAR WSN

CSCVH8-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAG GTB CAR CTG GTR SAG TCT

CSCVH9-F

GGT GGT TCC TCT AGA TCT TCC TCC TCT GGT GGC GGT GGC TCG GGC GGT GGT GGG GAG GTG CAA CTG VWG RAG TCY

Canine VH reverse primer

CSCG1234-B-T

CCT GGC CGG CCT GGC CAC TAG TAA CCG AGG GGG CCG TGG TGG A

Canine VLλ forward primers

CSCLam1a-F

GGG CCC AGG CGG CCG AGC TCG TGC TGA MTC MGC YRS SYT CD

CSCLam1b-F

GGG CCC AGG CGG CCG AGC TCR YSC TGA CTC ARM MGS CCT CM

CSCLam1c-F

GGG CCC AGG CGG CCG AGC TCG TSC TGA CTC AGC YDV CCT CA

CSCLam1d-F

GGG CCC AGG CGG CCG AGC TCG YGY TGA CYC ARC YRG CCT CM

CSCLam2-F

GGG CCC AGG CGG CCG AGC TCN BVY TGA CKC ARC CDD CCT CR

CSCLam3-F

GGG CCC AGG CGG CCG AGC TCG TGC TGW CWC AGC YGC CAT CM

CSCLam4-F

GGG CCC AGG CGG CCG AGC TCG TGC TGA CTC AGC CTC CYT C

CSCLam5-F

GGG CCC AGG CGG CCG AGC TCG RGY TGA CTC AGC YRC CWT C

CSCLam6-F

GGG CCC AGG CGG CCG AGC TCG GGY TGA ATC AGS CTY CCT C

CSCLam7-F

GGG CCC AGG CGG CCG AGC TCG TDN TVA CYC ARS MRV CMT CA

CSCLam8-F

GGG CCC AGG CGG CCG AGC TCT GCT GAC CCA GAC TCC AAG TG

CSCLam9-F

GGG CCC AGG CGG CCG AGC TCK CTG ACC CAG MCT MCA AGT GC

CSCLam10-F

GGG CCC AGG CGG CCG AGC TCG TRC TGA CYC ARC CKC CKT CW

CSCLam11-F

GGG CCC AGG CGG CCG AGC TCG TRM GSA AYC ARC CKC CKT CW

CSCLam12-F

GGG CCC AGG CGG CCG AGC TCC TGC TGA CYC ARC CKG CYT CW

CSCLam13-F

GGG CCC AGG CGG CCG AGC TCG TRC TGA AYC ARC CKC CKT CW

Canine VLλ reverse primers

CSCJLam1-B

GGA AGA TCT AGA GGA ACC ACC GCC GAG GAC GGT CAG STG GGT SCC

CSCJLam2-B

GGA AGA TCT AGA GGA ACC ACC ACC WAG GAC GGT SAG YTS GRT TCC

Canine VLκ forward primers

CSCK1-F

GGG CCC AGG CGG CCG AGC TCC AGA TGA CCC AGT CCC CAA

CSCK24-F

GGG CCC AGG CGG CCG AGC TCG TSA TGA YRC AGA CYC CAC

CSCK34-F

GGG CCC AGG CGG CCG AGC TCG TGA TGA CMC AGT CTC CAG

CSCK4-F

GGG CCC AGG CGG CCG AGC TCA YSM TGA CYC AGT KYC CAG

CSCK5-F

GGG CCC AGG CGG CCG AGC TCG TSA TGA YRC AGR CBC CAC

CSCK6-F

GGG CCC AGG CGG CCG AGC TCT GTC ATG ACA CAG ACC CCA

Canine VLκ reverse primers

 

CSCJK1-B

GGA AGA TCT AGA GGA ACC ACC TTT GAG YTC CAC CTK GGT WCC

CSCJK2-B

GGA AGA TCT AGA GGA ACC ACC TTT GAG CTC CTC CTT GGT TCG

CSCJK3-B

GGA AGA TCT AGA GGA ACC ACC TTT GAG GTC CAC CTT GGT TCC

CSCJK4-B

GGA AGA TCT AGA GGA ACC ACC TTT KAT CTC CAV CTT GGT YCC

Second round PCR primers for scFv construction

RSC-F

GAG GAG GAG GAG GAG GAG GCG GGG CCC AGG CGG CCG AGC TC

RSC-B-AA

GAG GAG GAG GAG GAG GAG CCT GGC CGG CCT GGC CAC TAG AA

Sequencing primers

OmpA

AAG ACA GCT ATC GCG ATT GCA G

DpSeq

AGA AGC GTA GTC CGG AAC GTC

Back to article page