Table 5 Biopanning selection strategy.

From: Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs

Round

Antigen

Input phage

Washing step

1

500 ng/well

100 µl/well x2 wells, 2 h

10 rounds, 5 min/round

2

500 ng/well

100 µl/well x2 wells, 2 h

10 rounds, 5 min/round

3

500 ng/well

100 µl/well, 2 h

5 rounds, 5 min/round

4

500 ng/well

100 µl/well, 2 h

5 rounds, 5 min/round

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