Fig. 5
From: Persistence of post-stress blood pressure elevation requires activation of astrocytes
Double fluorescent immunostaining of c-Fos (red) and S100 (green) with DAPI (blue) staining in the rostral ventral medulla (RVM). (a) Condition a without arundic acid pre-treatment and without stress loading; (b) Condition b: without arundic acid pre-treatment and with stress loading; (c) Condition c: with arundic acid pre-treatment and without stress loading; (d) Condition d: with arundic acid pre-treatment and with stress loading. Under Condition b, a number of c-Fos positive cells were observed in the superficial ventral medullary region, i.e., in the cardiovascular region RVM. Double positivity for c-Fos and S100 (yellow in color) was rare, as exemplified by a cell under Condition a (arrow). Bottom oblique line, ventral medullary surface. The anatomical portion of the depicted brain region is shown in Fig. S3. Scale bar 100 μm.
