Table 2 Sequences of primers for reverse transcription and quantitative polymerase chain reactions.
From: Implication of microRNAs as messengers of exercise adaptation in junior female triathlonists
Type | miRNA | Sequence |
|---|---|---|
Stem-loop | hsa-miR-133a-3p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAAC CAGCTG |
Stem-loop | hsa-miR-210-3p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAAC TCAGCC |
Stem-loop | hsa-miR-494-3p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAAC GAGGTT |
Stem-loop | hsa-miR-127-3p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAAC AGCCAA |
Stem-loop | hsa-let-7a-5p | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAAC AACTAT |
Stem-loop | U6 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATA |
Forward | hsa-miR-133a-3p | GTGTTTGGTCCCCTTCAAC |
Forward | hsa-miR-210-3p | GTTTCTGTGCGTGTGACAG |
forward | hsa-miR-494-3p | GGGTGAAACATACACGGGA |
Forward | hsa-miR-127-3p | GTTTTCGGATCCGTCTGAG |
Forward | hsa-let7a-5p | GGGTGAGGTAGTAGGTTGT |
Universal reverse primer for qPCR | CTGCAGGGTCCGAGGT | |
Upstream | U6 | CTCGCTTCGGCAGCACATA |
Downstream | U6 | GTGCAGGGTCCGAGGT |
