Fig. 2 | Scientific Reports

Fig. 2

From: Multiple cell types including melanocytes contribute to elastogenesis in the developing murine aortic valve

Fig. 2

Aortic valve cells clusters separately from other cardiac cells by spatial transcriptomic analysis at P3. (A) Spatial transcriptomics of P3 aortic valve sections. P3 aortic valve H&E image representation with surrounding tissue spots (blue) and aortic valve leaflet spots (red). Scale bar = 1 mm. (B) 2-D t-SNE cluster plot showing aortic valve leaflet tissue cluster (red) and surrounding tissue (blue). (C) List of top ten correlated genes with Eln from spatial transcriptomics data. For each spot (131 total), gene read count values were normalized to the total number of reads for a given spot. (D) Eln expressing cells are positive for alpha smooth muscle actin (αSMA). P0 aortic valve sections assessed by  in situ hybridization RNAscope for Eln (red), immunofluorescence for αSMA antibody (green) and nuclei counterstaining for DAPI (blue). Dotted white line outlines aortic valve leaflet. Scale bar = 10 μm. Yellow highlighted box corresponds to the zoom image showing high magnification of αSMA and Eln positive cells in the aortic valve (left). Scalebar = 5 μm. N = 6.

Back to article page