Fig. 3 | Scientific Reports

Fig. 3

From: Multiple cell types including melanocytes contribute to elastogenesis in the developing murine aortic valve

Fig. 3

Elastic fiber patterning in developing murine aortic valve. (A) Wholemount elastic (red) and collagen (cyan) fiber staining in WT (C57BL/6J mice), hyperpigmented (K5-Edn3) and hypopigmented (Albino) AoV leaflets at P0 and P7 stages. Leaflets in the light microscope images (inserts) correspond to those in the images of the stained leaflets. P0 images, 60X magnification and P7 images, 20X magnification. (B) Quantification of elastic fiber (left) and collagen fiber (right) staining at P0 (blue circle) and P7 (red circle) stages in WT, K5-Edn3 and Albino. (C) qPCR gene expression for elastin in K5-Edn3 and Albino at P0, P7 and P30 stages. P0 (blue circles), P7 (red circles) and P30 (black circles). Fold change normalized to endogenous Gapdh. Mean ± SEM shown; p-value determined by One-Way ANOVA followed by Tukey’s multiple comparison test; N = 5–9 biological replicates per group.

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