Fig. 5 | Scientific Reports

Fig. 5

From: Multiple cell types including melanocytes contribute to elastogenesis in the developing murine aortic valve

Fig. 5

Endothelial and interstitial cells express elastin mRNA except immune cells. 3-D reconstruction of P3 WT aortic valve wholemount leaflet labeled for elastin (Eln, magenta) in situ hybridization RNAscope probe, and antibody staining (green) for (A) VE-Cadherin (VE-Cad), (B) Vimentin (Vim), (C) CD45 and nuclei counterstaining for DAPI (blue). Yellow arrow heads points to co-localization. The yellow line shows the cross section corresponding to the orthogonal views. The XZ and YZ orthogonal views are shown in the bottom and side panels with only magenta and gray channel, respectively. N = 6. Scale bar = 50 μm. Wholemount single channels images are shown for each of the corresponding marker.

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