Fig. 3
From: Multicellular ovarian cancer spheroids: novel 3D model to mimic tumour complexity
Microscopic characterisation of the structure of simultaneously seeded mono and co-cultured spheroids. OvCar8 (OC), A2780 (OC) and Detroit 551 (Fibr.) monocellular and OvCar8 and Detroit 551, A2780 and Detroit 551 multi-cellular spheroids were cultured for 96 h as described in Fig. 2A. (A) Spheroids were examined by scanning electron microscopy after 96 h of growth. Scale bar 50 μm. (B,C) Prior to cultivation, the cells were stained with fluorescence dyes in order to subsequently assign them to a cell type during cultivation. These spheroids were fixed, cleared and imaged after 96 h of growth using LSM. Red/Cell Tracker Deep Red: ovarian cancer cells; green/CellTracker Green CMFDA: fibroblasts. Scale bar 200 μm. (D, E) Histological and immunohistochemical analysis of spheroid morphology and protein expression of OvCar8 (D) and A2780 (E) spheroids after 96 h of growth. Ki-67 is a proliferation marker, while PAX8 is marker for ovarian cancer cells and fibroblast activation protein markers fibroblasts, respectively. Scale bar 200 μm.
