Fig. 5
From: Multicellular ovarian cancer spheroids: novel 3D model to mimic tumour complexity
Growth kinetics of sequentially seeded co-cultured spheroids. (A) Workflow of the newly developed co-cultured spheroids with staggered seeding (with 24 h delay). OvCar8 and Detroit 551 cells, A2780 and Detroit 551 cells were seeded stained or unstained sequential (with 24 h delay) into ULA plates and grown for 96 h (from the time of the first seeding). Ovarian cancer cells were seeded first and then fibroblasts, so also vice versa (first seeded → second seeded). These reverse seeding processes were analysed and compared to each other. (B) Representative microscopic images of sequential seeded multi-cellular OvCar8-fibroblast spheroids after 96 h of growth. Scale bar 500 μm. (C) Quantitative differences in the size of different OvCar8-fibroblast spheroids after 96 h of growth. Quantitative data, t-test, *** (p < 0.001). (D) Representative microscopic images of sequential seeded multi-cellular A2780-fibroblast spheroids after 96 h of growth. Scale bar 500 μm .(E) Quantitative differences in the size of different A2780-fibroblast spheroids after 96 h. Quantitative data, t-test, * (p < 0.05), *** (p < 0.001), **** (p < 0.0001).
