Fig. 4 | Scientific Reports

Fig. 4

From: Efficacy of epidermal growth factor in suppressing inflammation and proliferation in pterygial fibroblasts through interactions with microenvironmental M1 macrophages

Fig. 4

The relative attenuation of inflammatory, fibrotic, and vasculogenic signals in pterygial fibroblasts by the delivery of EGF-preconditioned M1 macrophage-derived exosomes. (A) The low- and high-magnified images of the delivered PKH26 dye-labeled M1 macrophage-derived exosomes delivered and penetrated into the cultured pterygial fibroblasts. Scale bars: 200 μm (white) and 20 μm (black). (B-H) Real-time RT-PCR analysis for IL6, IL1B, MMP2, VCAM1, ICAM1, VEGFA, and VEGFC expressions in pterygial fibroblasts treated with M1 macrophage-derived exosome (M1 Exo, 20 µg/mL for 24 h) or EGF-preconditioned M1 macrophage-derived exosome (EGF-M1 Exo, 20 µg/mL for 24 h). Analysis of variance followed by Tuckey’s post-hoc test. N = 4. *p\(\:<\)0.05, **p\(\:<\)0.01, ***p\(\:<\)0.001, ****p\(\:<\)0.0001, and ns: not significant. EGF, epidermal growth factor; PF, pterygial fibroblast.

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