Fig. 2
The magnitude of effects of heat and Triton X-100 on the detection of IL-1α measured in percentage changes from the untreated samples. Paired aliquots of the same sample (connected with a line) were inactivated by exposure to 60 °C for 1 h (▲), or Triton X-100 (0.05%, ♦), or left untreated on ice. Some samples were unpaired due to unavailability of sample to be tested in all three conditions. The magnitude of effects on IL-1α detection in percentage was tested against a hypothetical value ‘0’ (dashed line on the graph) using “Wilcoxon Signed Rank Test”. The dashed line represents ‘0%’ deviation from the detected level of cytokine/chemokine in the untreated samples and is calculated as [(Streated−Suntreated Ctrl)/ Suntreated Ctrl × 100%].The p-values were shown near x-axis. The difference in the magnitude effects between the two inactivation-methods was tested using "Wilcoxon matched-pairs signed rank test". ‘*’p < 0.05, ‘**’p < 0.005.
