Table 1 Assay validation parameters of the proposed HPLC method for determination of Paracetamol, Dexketoprofen Trometamol and Rivaroxiban.

From: A sustainable and green HPLC-PDA technique for the simultaneous estimation of Post-COVID-19 syndrome co-administered drugs with greenness and whiteness assessment

Parameter

PAR

DEX

RIV

Linearitya (µg/mL)

3.00–45.00

0.5–50.00

0.15-20.00

Slope

0.1268

0.0978

0.1181

Intercept

-0.0257

-0.0014

0.0016

Correlation coefficient(r)

1

1

1

Accuracya (Mean ± SD)

101.20 ± 0.912

100.45 ± 1.374

100.21 ± 0.850

Intra-dayab (RSD %)

0.318

0.583

0.193

Inter-dayab (RSD %)

0.988

0.860

0.979

Robustness (RSD %)*

0.410 c

0.325 d

0.061 c

1.570 d

0.514 c

0.086 d

LOD(µg/mL)e

0.531

0.095

0.047

LOQ(µg/mL)f

1.608

0.289

0.143

  1. aMean of three determinations.
  2. bRelative standard deviations for concentrations (20.00, 25.00 &35.00 µg/mL) for PAR, (13.00, 20.00 & 30.00 µg/mL) for DEX and (4.00, 10.00 & 20.00 µg/mL) for RIV.
  3. cSmall deliberate changes in the flow rate (1.5 ± 0.1 mL/min), the tried flow rates were (1.4, 1.5 and 1.6 mL/min).
  4. dSmall deliberate changes in wavelength of detection (± 2 nm) were made. The tried wavelengths were 252.0, 254.0 and 256.0.
  5. eLOD = 3.3 × SD of intercept/slope.
  6. fLOQ = 10 × SD of intercept/ slope.
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