Fig. 1

Gut microbiome analysis in two mouse models of chronic pancreatitis. A, B Azan blue staining of pancreatic tissue of caerulein induced CP and T7D23A heterozygous mice. Faecal samples from colon and duodenum were collected from C57Bl/6-J mice after CP induction (n = 8), from T7D23A mice (n = 11) and from control animals (n = 39). Isolated DNA was analysed by 16 S rRNA gene sequencing. C Principal coordinate analysis illustrates the changes of gut microbiome between controls and CP mice in colonic samples (R² = 14.14%, p ≤ 0.001), a bar graph illustrates the 20 taxa with the highest fold change in the relative abundance of the CP group in relation to controls. D PCoA (R² = 7.00%, p = 0.01) and bar graph illustrate the changes in the duodenal microbiome. E, F PCoA illustrate the microbiome in colon (R² = 7.17%, p ≤ 0.001) and duodenum of T7D24A mice compared to controls (R² = 15.21%, p ≤ 0.001). Bar graphs showed the most affected taxa. Significant changes were tested by PERMANOVA.