Fig. 2

Gut microbiome analysis in two mouse models of acute pancreatitis. A, B H&E staining of pancreatic tissue after AP induction by caerulein and severe AP (SAP) induction by pancreatic duct ligation. Faecal samples from colon and duodenum were collected from C57Bl/6-J mice after AP induction (n = 15), from SAP mice (n = 44) and from control animals (n = 39). Isolated DNA was analysed by 16 S rRNA gene sequencing. C, D Principal coordinate analysis illustrates gut microbiome changes between controls and AP mice in colonic samples (R² = 8.88%, p ≤ 0.001) (C) and duodenum samples (R² = 4.77%, p = 0.029) (D). Bar graphs show the 20 most up-or downregulated taxa. E, F PCoA illustrates the microbiome changes of SAP mice compared to controls in colon (R² = 23.78%, p ≤ 0.001) and duodenum (R² = 31.52%, p ≤ 0.001), bar graphs show the 20 most affected taxa. Significant changes were tested by PERMANOVA.