Fig. 3 | Scientific Reports

Fig. 3

From: Inhibition of NLRP3 inflammasome contributes to paclitaxel efficacy in triple negative breast cancer treatment

Fig. 3

Expression of NLRP3, ASC, IL-1β, caspase-1 and caspase-3 protein in normal and tumor cells treated with PTX. Confocal immunofluorescence microscopy images revealing (a) NLRP3 and ASC, (b) NLRP3 and IL-1β, (c) NLRP3 and caspase-1, (d) NLRP3 and caspase-3 protein expression in MCF-12A and MDA-MB-231 cells before and after treatment with 100 nM PTX for 24 h (scale bar 50 μm). NLRP3 is shown green (AF488), ASC, IL-1β, caspase-1 and caspase-3 in red (AF546) and cell nuclei in blue (Hoechst 33342). (e, f, g, h) Quantitative measurement of fluorescence intensity was performed with ImageJ software. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

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