Fig. 4
The effects of NLRP3 pharmacological modulation on gene expression. Differential expression in MCF-12A and MDA-MB-231 cells of (a) NLRP3, (b) ASC, (c) AIM2, (d) NOD2, (e) IL-18, (f) IL-1β, (g) caspase-1, (h) GSDMD and (i) GSDME genes. MCF-12A and MDA-MB-231 cells were primed with 1 µg/mL LPS for 4 h followed by treatment with 5 mM ATP for an additional 1 h and then exposed to 10 µM MCC950 and/or 100 nM PTX for 24 h. Statistical significance: “*” symbol was used for statistical comparisons between cell lines; “#” symbol was used for statistical comparisons between experimental condition; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001 and #### p < 0.0001 (n = 3, biological replicates).
