Fig. 5
Multiplexing the Lumit Human IL-1β Immunoassay with the Caspase-Glo 1 Inflammasome Assay. (a) MCF-12A and (b) MDA-MB-231 cells were primed with 1 µg/mL LPS for 4 h followed by treatment with 5 mM ATP for an additional 1 h and then exposed to 10 µM MCC950 and/or 100 nM PTX for 24 h. Statistical significance: “*” symbol was used for statistical comparisons between the levels of caspase-1 secreted in each experimental condition; “#” symbol was used for statistical comparisons between the levels of IL-1β secreted in each experimental condition; ** p < 0.01, *** p < 0.001, **** p < 0.0001, ### p < 0.001 and #### p < 0.0001 (n = 3, biological replicates).
