Fig. 8 | Scientific Reports

Fig. 8

From: Inhibition of NLRP3 inflammasome contributes to paclitaxel efficacy in triple negative breast cancer treatment

Fig. 8

NLRP3 inflammasome – related protein expression in NLRP3 knock-down cells before and after exposure to 100 nM PTX. Confocal immunofluorescence microscopy images revealing (a) NLRP3, ASC, (b) caspase-1 and (c) IL-1β protein expression in MDA-MB-231 cells before and after NLRP3 siRNA-mediated knock-down and 100 nM PTX treatment (scale bar 50 μm). NLRP3 is shown green (AF488), while ASC, IL-1β and caspase-1 in red (AF546) and cell nuclei in blue (Hoechst 33342). (D, E) Quantitative measurement of fluorescence intensity was performed with ImageJ software. Statistical significance: ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

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