Fig. 3

Ability of vitrified-warmed fertilized oocytes derived from in vivo and in vitro fertilization to reach each development stage. To examine the developmental ability of fertilized oocytes in SD (A) and F344 (B) strains, PN oocytes derived from in vivo and in vitro fertilization were cultured in mR1ECM. The developmental rates of each stage were calculated as the number of embryos in each stage divided by the number of cultured pronuclear stage oocytes × 100. Fertilized oocytes in the F344 strain derived from in vivo and in vitro did not develop into blastocysts. *Values are significantly different in the comparison between in vivo and in vitro (P < 0.05).