Fig. 1 | Scientific Reports

Fig. 1

From: The Atlantic Cod MHC I compartment has the properties needed for cross-presentation in the absence of MHC II

Fig. 1

Atlantic cod MHC I colocalizes more with late endosomal and lysosomal markers than early and recycling endosomal markers in ACL cells. (A) Representative image of an ACL cell transiently co-transfected with MHC I-GFP, mCherry-Rab5 and BFP-Rab7A. Cells were imaged using a Zeiss LSM880 Fast AiryScan microscope. Scale bar: 10 μm. Magnifications of boxed areas 1 and 2 are shown below. (B) Representative image of an ACL cell transiently co-transfected with MHC I-GFP and mApple-Rab11. Cells were imaged using a Zeiss LSM880 Fast AiryScan microscope. Scale bar: 10 μm. Magnifications of boxed areas 1 and 2 are shown below. (C) The graph represents colocalization analysis between Atlantic cod MHC I-GFP and the indicated endosomal and lysosomal markers. Colocalization was analysed by calculating Manders’ coefficient in ImageJ. Scatter plot shows the mean ± SEM from three independent experiments, colour coded for each experimental repeat. Dots represent individual measurements and are colour coded according to experimental repeat. n > 32 cells.

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