Fig. 4

Egg-laying assay. The top diagrams display the viable offspring (ordinate) counted for the given day (abscissa) (A–D). The overall number of viable offspring (area under the curve) is presented in the column diagram below (E–G). Data for the four strains N2 (A), mtl-1 (B), 10md-MT (C) and 2d-MT (D) are shown. These data are further characterized by different concentrations of Cd²⁺: (E) 0 µM Cd²⁺, (F) 2.5 µM Cd²⁺, and (G) 12 µM Cd²⁺. Statistical analysis included technical replicates: N2: 5 experiments; mtl-1: 8 experiments; 10md-MT: 5 experiments; 0 µM: N2: 16 worms, mtl-1: 11 worms; 10md-MT: 19 worms; 2d-MT; 13 worms; 2.5 µM: N2: 16 worms; mtl-1: 9 worms; 10md-MT: 23 worms; 2d-MT; 15 worms; 12 µM: N2: 18 worms; mtl-1: 12 worms; 10md-MT: 20 worms; 2d-MT; 18 worms. Error bars indicate the standard error at 95% confidence. Significance is marked by asterisks.