Fig. 3
Antibody response to vaccination in the mouse model. A Timeline of inoculation (time points indicated by syringe cartoons), bleeding (depicted by red droplets), and harvesting of splenocytes (scalpel). Letter(s) next to each icon indicate(s) related panels. After one month, three mice from control (-), spike (S), liposome: spike (L: S), and spike: ferritin (S: F) groups were sacrificed to obtain fresh splenocytes for spike IFN quantification. The remaining mice were kept for an additional month. B, C Antibody titers of sera from mice inoculated with each immunogen for 2 weeks (B) and 1 month (C) after the first inoculation. D Pseudovirus neutralization titers from sera collected one month after the first dose. E Spike IFN levels one month after the first dose. F Effect of adjuvant on antibody titers (left) and on pseudovirus neutralization (right) 2 months after first inoculation for selected immunogens. Horizontal lines indicate mean values. Mean values of immunogens of the same dose, doses of the same immunogen, and immunogens with and without adjuvant were compared using the Bonferroni test. Asterisks indicate statistical significance. *p < 0.0332; **p < 0.0021; ***p < 0.0002; ****p < 0.0001. S, spike; L:S, liposome: spike; R, spike rosettes; S:F, spike: ferritin
