Fig. 1

Characteristics of IVP and EUS-EMG activity during reflexive urination. (A) Representative bladder pressure trace (top); and time-locked EUS-EMG signal (bottom). The latency refers to the time between the sudden rise in bladder pressure (threshold pressure) and the onset of EUS-EMG activity. ∆P represents the pressure difference between the threshold pressure (the first circular black dot) and the end pressure (the second circular black dot). (B) Total duration of representative EMG data including the bursting phase (EUS bursting activity duration, T1) and the tonic phase (tonic activity duration, T2). The bursting (phasic) pattern of activity (T1) produces the pulsatile release of urine. An enlarged representation of the T1 period (bottom), including the bursting EUS contraction period (active period, AP) and the EUS relaxation period (silent period, SP). (C) Quantification of the duration of T1 and T2. n = 12 mice. (D) Quantification of the maximum amplitude of EMG signals. n = 12 mice. (E) Quantification of the duration of AP and SP. Student’s t-test (two-tailed, paired). ***p < 0.001 n = 12 mice. (F) Quantification of the total number of active phases (APs) in each EMG data during the EUS bursting phase. n = 12 mice.