Fig. 4
High-level spinal cord injury caused NLUTD. (A) Schematic of the void spot assay setup. (B) Examples of void spots from sham mice (left) and SCI mice (right) illustrating impaired urination function. (C–E) Quantification of the interval for each void (C), the average area of each void spot (D), and the average diameter of each void spot (E). n = 12 mice for each group. Student’s t-test or Mann-Whitney U test (two-tailed, unpaired). ***p < 0.001. (F) Diagram depicting cystometry combined with external urethral sphincter (EUS) electromyography (EMG) recording using a multi-channel physiological recording instrument. (G) Representative intravesical pressure (IVP) traces (top) and time-locked EUS-EMG signals (bottom) during continuous transvesical infusion cytometry in anesthetized SCI mice or sham mice. Each urination event is denoted with an asterisk. Non-urination events are denoted with an arrowhead. Intravesical pressure data were subjected to Savitzky–Golay filtering in panel (G). (H) Enlarged view of the dashed line boxes in panel (G).
