Table 1 Evaluation of mRNA stability before and after optimization.

From: A comprehensive and single-use foot-and-mouth disease sero-surveillance prototype employing rationally designed multiple viral antigens

Sequences

Minimum free energy (RNAfold) before optimization (kcal/mol)

Minimum free energy (RNAfold) after optimization (kcal/mol)

Free energy of the thermodynamic ensemble (RNAfold) before optimization (kcal/mol)

Free energy of the thermodynamic ensemble (RNAfold) after optimization (kcal/mol)

UNAFold Mfold initial ΔG before optimization (kcal/mol)

UNAFold Mfold initial ΔG after optimization (kcal/mol)

B4 (VP1-O)

 − 228.80 

 − 253.60 

 − 237.34 l

 − 261.78 

 − 233.10 

 − 259.60

B5 (VP1-A)

 − 219.70 

 − 265.00 

 − 227.05

 − 273.72 

 − 229.20 

 − 271.80 

B6 (VP1-Asia1)

 − 222.40 

 − 260.80

 − 231.22

 − 269.39 

 − 231.90

 − 265.40

P2 (VP2)

 − 237.00 

 − 248.50 

 − 247.65 

 − 255.30

 − 248.40 

 − 252.90 

P3 (3AB)

 − 228.00 

 − 229.20 

 − 238.64 

 − 239.34 

 − 243.60 

 − 241.60

  1. The table lists the minimum free energy and free energy of thermodynamic ensemble of the mRNA secondary structure of the 5 sequences (B4, B5, B6, P2, P3) of study before and after their codon optimization. The more negative energy after optimization in each case denotes greater stability. The results coincided with the ΔG values also which also turned more negative after optimization except for P3.
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