Fig. 1
Podocytes respond to ANP/pGC stimulation with a long-lasting cGMP increase. Acute kidney slices obtained from (a) Tie2:Cre/cGi500 mice or (d) Pod: Cre/cGi500 mice showing cell-specific cGi500 biosensor expression with overlapping CFP/YFP fluorescence patterns in GECs and podocytes. (b, e) AKS were superfused (dotted lines) from 200 s to 570 s with 1 µM ANP (blue trace), 1 mM SNAP (orange trace), or the combination of both (black trace). Stimulation was followed by a washout phase with 1X KHB (37 °C). Time-lapse recordings for each cell type represent the mean of baseline-normalized FRET (CFP/YFP) ratios (\(\:\Delta\)R/Ro) of all analyzed glomeruli. Relative signal changes in ∆R/Ro (%) reflect changes in the intracellular cGMP concentration. (c, f) Scatter dot plots display the maximal ∆R/Ro response for each glomerulus derived from the indicated stimulation of (b) Tie2:Cre/cGi500 mice or (e) Pod:Cre/cGi500 mice during the entire measurement period of 1500 s. Data represent mean ± SEM of at least 9 glomeruli per condition obtained from slices of six Tie2:Cre/cGi500 mice and five Pod:Cre/cGi500 mice. One-way ANOVA, Tukey´s post hoc test, *P < 0.05. Scale bar 25 µM.
