Fig. 6
Functional assessment of NK cells post-production and activation. The luminescence-based assays compare the cytotoxic response of non-activated NK cells (‘non-act’) and 12-h cytokine activated NK cells (‘act’) against (a) K562 and (b) SH-SY5Y NBL targets at different effector to target ratios. Additionally, the P_NK3_act sample underwent post-activation characterization after a freeze/thaw cycle, denoted by an asterisk*. The P_NK1_act* sample was exclusively assessed post-freeze/thaw, with no corresponding non-activated control for comparison. The reported percentage of cell lysis represents the average of five replicates, with standard deviation (SD) indicated by error bars. c) The cytotoxicity of cytokine-activated NK cells targeting AML blast cells from patient samples. d) The degranulation response of NK cells following a 4-hour co-culture with K562 and SH-SY5Y cell lines, compared to NK cells cultured in the media alone (n = 2). P_NK: NK cells were produced in the GMP CliniMACS Prodigy conditions. R_NK and non-activated NK cells were produced in a non-GMP conditions. AML blast_P denotes AML patient.
